sequence of blood draw tubes
The recommended order of draw is as follows. Necessitating the change was the industry-wide transition from glass blood collection tubes to plastic.
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3 mL no additive must be used ONLY if drawing a citrate blue tube.
. CANNOT be collected via capillary collection method. The draw order for specimen tubes is as follows. When collecting more than one tube type always.
This order of draw has changed over the years the last occurring in 2003. They are listed by chemical additives that have been introduced to the tube by the manufacturer to perform a specific function when mixed with the blood. Blood Cultures Sterile Tubes.
Blood Gas Syringe EDTA tubes Sodium Citrate tubes all other anticoagulant tubes SerumClot tubes. In phlebotomy this term has special meaning as it is the order in which certain tubes can be used. Dark green tubes are both Sodi-um and Lithium heparin.
Draw Royal Blue top tubes in the order indicated by the additive. Lavender Tube contains K2EDTA additive used for hematology testing. Do not freeze whole blood unless specifically indicated by the specimen requirements.
Cross-contamination of tube additives resulting in spurious results eg. Blood Cultures 8 to 10 times Serum glass tube Citrate 3 to 4 times BD SST Gel Separator Tube 5 times BD SST Gel Separator Tube Serum plastic tube Heparin 8 to 10 times BD PST Gel Separator Tube With Heparin EDTA 8 to 10 times Fluoride glucose tube 8 to 10 times BD Vacutainer Systems Preanalytical Solutions 1 Becton Drive. When collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on Clinical and Laboratory Standards Institutes CLSI standard H3-A6 from November 2007 as follows.
Gold SST Plain tube wgel and clot activator additive Green and Dark Green Heparin with and without gel Lavender EDTA Pink - Blood Bank EDTA Gray OxalateFluoride List Updated 02112019. Phlebotomy Tubes and Order of Draw. 1 Blood cultures yellow SPS sterile 2 Light blue buffered sodium citrate tube 3.
Tube MUST contain venous blood only. Order of blood draw. 9 rows Blood Draw Order Tube ColorDescription ShowHide All.
Blood Cultures Invert 8 to 10 times 2. Color Varies Blood Cultures. Purple top tubes should be 50-60 full.
Standard order of draw. Siphoning the bloods directly into the tubes the Order of Draw in order is. Blue top Tube contains sodium Citrate.
Do not freeze glass tubes. Do not package frozen specimens with non- frozen specimens. Pour off serum or plasma into a properly labeled plastic tube before freezing.
The list below lists the most commonly used blood collection tubes their additives and uses in laboratory. Filling up the clean blood culture tubes first followed by filling of coagulation tubes for accurate analysis of the coagulation time then non-additive serum tube or serum. Given a scenario and some possible suspects students will perform a series of tests.
Blood culture bottle non-additive tube coagulation tube clot activator serum separator tube sodium heparin plasma separator tube ethylenediaminetetraacetic acid. Collected first to obtain adequate volume minimize platelet clumping. The correct order of draw follows.
Sodium EDTA contamination resulting in low calcium and high sodium. Specimens must remain frozen during shipment. Whereas glass is a natural clot activator plastic is not.
DRAW ORDER TUBE TOPSTOPPER COLOR TUBE TYPE 1 Blood cultures 2 Light Blue Citrate 3 RedGoldTiger Serum 4 Green Heparin 5 Lavender Oxalate EDTA 6 Gray Here is a mnemonic device to help you remember. With a tube holder and butterfly needle. Citrate Tube Light Blue SST TM Gel Separator Tube Red or Gold or Serum Tube Plastic or Glass - Red PST TM Gel Separator Tube Green or Heparin Tube Sodium or Lithium - Green EDTA Tube Lavender Fluoride Tube Grey.
7 mL No additive - Red strip on label - Draw as 4 6 mL K2-EDTA - Blue strip on label - Draw as 7. The correct order of draw follows. Nowadays the order of blood draw established by the international guidelines is postulated to avoid cross-contamination between different additives contained in blood collection tubes and it is as follows.
Invert 8 to 10 times. Blood culture tube or bottle Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures Heparin tube with or without gel eg. Blood culture tube or bottle.
Gently mix all tubes as they are collected by inverting each tube 5-8 times. Capillary tubes Used for whole blood tests andor point of care testing blood gasses. Most blood collection tubes contain an additive that either accelerates clotting of the blood clot activator or prevents the blood from clotting anticoagulant.
There are seven tubes which make up the order of draw. Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures Heparin tube with or without gel eg. So in order for blood to clot in safer plastic tubes manufacturers coat the inside of the tube with a.
Used only to remove air from tubing. 3 mL no additive must be used ONLY if drawing a citrate blue tube. Monterey CA 93940 Blood Tube Selection and Order of Draw Blood Tube Selection and Order of Draw 1.
13 rows Collect the blood into the containers in the order listed below for evacuated systems. 8 rows Blood Draw Order Tube Color NameAdditive of Inversions. Order of Draw When Using Evacuated Tubes.
The flow of blood needs to be steady but not forced when filling tubes. The order to fill blood tubes is. Order of blood draw and color-coded tops for blood draw 38 cards 2020-05-22 6.
Thus the order of draw follows the broad principles of. Blue tube for coagulation Sodium Citrate Red No Gel. In general tests requiring whole blood call for blood samples collected in test tubes containing some form of the anticoagulant EDTA.
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